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RESEARCH ARTICLE

4-Hydroxy-2 (or 5)-ethyl-5 (or 2)-methyl-3 (2H)-furanone의 미백 활성 효과

원문정보

Inhibitory effect of 4-hydroxy-2 (or 5)-ethyl-5 (or 2)-methyl-3 (2H)-furanone on Melanogenesis

장혜인, 고혜정, 한영숙, 김재희

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The aim of this study was to investigate whitening effect of 4- hydroxy-2 (or 5)-ethyl-5 (or 2)-methyl-3 (2H)-furanone (HEMF). The antioxidant activity of HEMF was evaluated using in vitro 1,1- diphenyl-2-picrylhydrazy (DPPH) free radical assay. Inhibitory effects of HEMF against tyrosinase activity and melanogenesis in B16 melanoma cells were assessed in vitro to determine whitening effect. HEMF showed strong radical scavenging activity in DPPH assay. The SC50 (concentration that inhibits 50% of the DPPH radical) values of HEMF was 1.5 mg/L. 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay with B16 melanoma cells showed that HEMF was not toxic up to the concentration of 1000 mg/L. HEMF induced dose-dependent inhibition of mushroom tyrosinase activity showing 71% inhibition at a concentration of 250 mg/L. Various concentrations (2.5-50 mg/L) of HEMF also significantly inhibited tyrosinase activity in B16 melanoma cells by 20-65 %. In addition, HEMF significantly reduced melanin contents of B16 melanoma cells by 52 % at a concentration of 50 mg/L. These results suggest that HEMF could be an effective whitening agent.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 시료 및 기기
  2. 세포주 및 세포배양
  3. 자유 라디컬 소거력 측정
  4. 세포 생존력 측정
  5. Mushroom tyrosinase 활성 억제 실험
  6. B16 세포에서 tyrosinase 활성 억제 실험
  7. B16 세포에서 melanin 생성 억제 효과 실험
  8. 통계처리
 Ⅲ. 연구결과 및 고찰
  1. 자유라디컬 소거력 측정
  2. 세포 생존력 측정
  3. Mushroom tyrosinase 활성 억제 실험 결과
  4. B16 melanoma세포에서 tyrosinase 활성 억제 실험 결과
  5. B16 melanoma 세포에서 멜라닌 생성 억제 효과 실험 결과
 Ⅳ. 결론
 감사의 글
 참고문헌

저자정보

  • 장혜인 Hye-In Jang. 경기대학교 대체의학대학원
  • 고혜정 Hye-Jung Koh. 수원여자대학교 미용예술학과
  • 한영숙 Young-Sook Han. 동남보건대학 피부미용과
  • 김재희 Jaehee Kim. 경기대학교 대체의학대학원

참고문헌

자료제공 : 네이버학술정보

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