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논문검색

Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry

원문정보

C. S. Park, Z. H. Li, N. D. Sung, D. I. Jin, P. Q. Cong, E. S. Kim, E. S. Song, Y. J. Yi

한국동물생명공학회(구 한국동물번식학회) Reproductive & developmental biology Volume 29 No 4 2005.12 pp.253-257
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than 80% of fresh sperm washed with mTLP-PVA medium at 20℃ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane (36.4~46.9%) and nonfunctional mitochondrion (55.1~71.1%) in the mTLP-PVA and BTS washing media at 20℃. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at 4℃ washing temperature than at 20℃ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

목차

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자정보

  • C. S. Park Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • Z. H. Li Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • N. D. Sung Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • D. I. Jin Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • P. Q. Cong Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • E. S. Kim Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • E. S. Song Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University
  • Y. J. Yi Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University

참고문헌

자료제공 : 네이버학술정보

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