원문정보
초록
영어
A gene encoding carboxymethylcellualse (CMCase) from a marine bacterium Bacillus velezensis A-68 LBH-14 had been cloned into E. coli JM109. The optimal carbon and nitrogen sources for cell growth of a recombinant E. coli JM109/A-68 were found to be maltose and tryptone, whereas those for production of CMCase were rice bran and yeast extract. Based on productivity and economic aspect, rice bran and ammonium chloride were chosen to carbon and nitrogen sources for optimization of major conditions for enhanced production of CMCase using response surface method (RSM). The optimal conditions for rice bran, ammonium chloride, and initial pH of medium for cell growth were found to be 150.0 g/L, 2.5 g/L, and 8.0, whereas those for production of CMCase were 125 g/L, 5.0 g/L, and 7.0. The maximal cell growth and production of CMCase by E. coli JM109/A-68 were predicted as 8.20 g/L and 1079.7 U/mL.