earticle

영어

Cellulophaga lytica LBH-14, w hich had been isolated from seawater in the previous study, was known to produce two types of cellulases, carboxymethylcellulase (CMCase) and cellobiase. The optimal carbon and nitrogen sources for cell growth of C. lytica LBH-14 were found to be rice hulls and yeast extract, whereas those for production of CMCase were rice bran and yeast extract. Based on productivity and economic aspect, rice bran and ammonium nitrate were chosen to carbon and nitrogen sources for optimization of major conditions for enhanced production of cellulases. The optimal conditions of rice bran, ammonium nitrate, and initial pH of medium for cell growth were 125 g/L, 5.0 g/L, and 6.5, whereas those for production of CMCase and cellobiase were 100 g/L, 6.5 g/L, and 6.5. The optimal conditions for production of CMCase extracted using orthogonal array method (OAM) were the same as those of cellobiase. However, the optimal conditions of rice bran, ammonium nitrate, and initial pH of medium for production of CMCase were predicted as 75.0 g/L, 7.5 g/L, and 6.5 using response surface method (RSM), whereas those for production of cellobiase were 88.1 g/L, 4.9 g/L, and 4.7. The maximal cell growth and production of CMCase and cellobiase by C. lytica LBH-14 were predicted as 8.91 g/L, 350.1 U/mL, and 121.1 U/mL, respectively.