earticle

논문검색

학생구두발표 (석사과정)

Effect of Carbon Source and Plant-derived Peptone on hCTLA4Ig Production in Transgenic Rice Cell Cultures

원문정보

Gi-Hong JIN, Jun-Young KWON, Hyung-Jin NAM, Myoung-Sik KIM, Jun-Yong PARK, Sun-Hee JEONG, Yun-Young PAK, Min-Sub KIM, Hong-Yeol CHOI, Dong-Il KIM

한국생물공학회 한국생물공학회 학술대회 2012 추계학술대회 2012.09 pp.49-49
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Plant-derived peptones (PPs) have been utilized to improve cell growth and volumetric productivity in plant cell cultures. PPs contain amino acids, carbohydrates and minerals. Carbon (C) and nitrogen (N) sources have been known as major factors in plant cell cultures. Supplementation of chemically defined medium with PP was found to enhance cell growth and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) significantly in transgenic rice cell suspension cultures. Therefore, it was necessary to investigate the effects of C and N sources for the improvement of recombinant protein production. The concentrations of C and N sources used in this study were as follows: 0 (control), 1, 3, 5% (w/v) of sucrose and 10, 20, 30 g/L of PP. In case of adding 3% sucrose with 10 g/L of PP, maximum dry cell weight was 1.9-fold higher than that of control and the maximum level of hCTLA4Ig reached up to 64.8 mg/L. Whereas hCTLA4Ig was not produced in control cultures. In conclusion, it was found that the determination of optimal concentration ratio between C and N sources are important to improve cell growth and hCTLA4Ig production in transgenic rice cell cultures.

키워드

저자정보

  • Gi-Hong JIN Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Jun-Young KWON Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Hyung-Jin NAM Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Myoung-Sik KIM Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Jun-Yong PARK Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Sun-Hee JEONG Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Yun-Young PAK Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Min-Sub KIM Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Hong-Yeol CHOI Dept. of Biological Engineering, Inha University, Incheon, 402-751.
  • Dong-Il KIM Dept. of Biological Engineering, Inha University, Incheon, 402-751.

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.