earticle

영어

The approach of nanoscale enzyme reactors (NERs), consisting of enzyme adsorption into mesoporous materials with a bottle-neck structure and follow-up enzyme crosslinking, have demonstrated successful stabilization of enzyme activity in a ship-in-a-bottle mechanism. In details, crosslinked enzymes in larger mesocellular pores cannot leach out through smaller bottle-neck mesopores.1 When compared to simple adsorption with no enzyme crosslinking, the NER approach has proven to be effective in improving the stability and sensitivity of electrochemical biosensors. 2 For example, glucose oxidase (GOx) and organophosphorus hydrolase (OPH) were immobilized and stabilized in mesoporous carbons (MSU-F-C) with mesocellular pores (26 nm) connected by window mesopores (17 nm). NERs of GOx and OPH in MSU-F-C were employed to develop highly stable and sensitive enzyme-based biosensors, which can find its potential applications in medical and environmental biosensors.