earticle

영어

Lipase (LP) is one of well-known enzymes for a wide range of applications such as detergents, foods, oils and cosmetics and pharmaceutical industries. In the present work, LPs were immobilized and stabilized into polyaniline nanofibers (PANFs) via a three-step process of enzyme adsorption, precipitation and crosslinking (EAPC). As a result, EAPC retained high enzyme loading and showed highly-enhanced enzyme stability. In other words, EAPC showed 5.1 and 5.9 times higher activity than enzyme adsorption (EA) and enzyme adsorption/crosslinking (EAC), respectively. After 84 days under 200 rpm shaking at room temperature, the residual activities of EA, EAC and EAPC were 11%, 24% and 74% of initial activities, respectively. The esterification of ibuprofen with n-propoanl in isooctane was performed by using EA, EAC and EAPC, and the different amounts of dioctyl sodium sulfosuccinate (aerosol-OT, AOT) were added to better disperse the immobilized enzymes. The addition of 100 mM AOT resulted in 57-fold increase of EAPC activity when compared to the case with no AOT addition. Highly-stable and active EAPC showed a great potential of successes in various enzyme applications.