earticle

영어

It becomes important to develop novel biotechnologies that can subside the global climate change problem, mainly revoked by emission of carbon dioxide (CO2). Since carbonic anhydrase (CA, EC 4.2.1) accelerates the hydration of CO2, CA-based CO2 capture tool is considered as one of the potential systems for solving the problems. Recently, we cloned several types of CAs from marine microalgae or marine bacteria and we re-constructed their sequences optimized for highly-efficient production in E. coli system. Several CAs were obtained successfully in microbial expression system and their properties were characterized in terms of their CO2 sequestration ability. In particular, the acquired CAs could mediate catalysis of the dissolved CO2 to bicarbonate (HCO3 -), which can be precipitated as CaCO3 in the presence of Ca2+. CA-based CO2 mineralization can be used as potent platform technology for CO2 capture and use. Furthermore, HCO3 - mediated by CAs can be attached with PEP (phosphoenolpyruvate) by phosphoenolpyruvate carboxylase (PEPCase, EC 4.1.1.31) to make four-carbon organic acid, oxaloacetate (OAA). These approaches based on marine CA will be platform system for development of efficient CO2 capture/use technologies.