earticle

영어

Homogeneous immunoassays are attractive analytical technologies in food and environmental analyses because they are superior to heterogeneous immunoassays in sample and reagent consumption, analysis time, portability and disposability. Herein, we have developed fluorescence resonance energy transfer (FRET) homogeneous immunoassay for the detection of mycotoxins, such as aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN). Owing to intrinsic fluorescence properties of protein and mycotoxins, antibody and antigen played roles as the respective donor and acceptor in the FRET immunoassay. The FRET immunoassay, based on intact antibody, displayed high specificities and detection limits of 1 and 0.85 ng mL-1 for OTA and AFB1, respectively. Furthermore, the sensitivity of Fab fragment-based FRET immunoassay was obtained 10-fold enhancement comparing to the intact antibody-based immunoassay. Practical applications of the FRET immunoassay system were demonstrated by their use in the detection of OTA or AFB1 in spiked grain samples. The observations made in these efforts show that the homogeneous FRET immunoassay strategy, which requires a simple sample preparation procedure, serves as a powerful tool for the rapid and sensitive quantitative determination of mycotoxin in food and environmental samples.