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RT-PCR 이용 난지형 마늘 주산지 바이러스 감염실태 조사

원문정보

Survey on Viral Infections of Southern Type Garlic Using Reverse Transcription-Polymerase Chain Reaction

최학순, 정봉남, 곽용범, 정명일, 장석우, 양은영, 조명철, 정승룡

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

In early April 2008, garlic leaves were sampled from 5 areas for virus detection. The detection of viruses such as OYDV (onion yellow dwarf virus), LYSV (leek yellow stripe virus), GLV (garlic latent virus) and GarVs was done using RT-PCR. The primers were synthesized to amplify the coat protein genes of these viruses (Sumi and Ayabe, 2001). OYDV and LYSV, the viruses which are known to greatly decrease the yield of garlic bulbs, infected 100% of the garlic in all five areas. GarVs was also observed in 100% of the garlic from all five areas. A portion of subjects did not show mosaic symptom that is visible to the naked eye, however, were infected with a virus. 100% of garlic from Haenam and Muan was infected, while 93.8% from Namhae, Goheung and Shinan were infected with GLV. GLV is a carlavirus that shows undetectable effects alone but causes critical damage when it co-infected with potyvirus.

목차

Abstract
 서론
 재료 및 방법
  시험재료
  RNA 추출 및 프라이머 정보
  cDNA 합성 및 RT-PCR
 결과 및 고찰
  주아재배 연차간 RT-PCR 이용 바이러스 검정
  난지형 마늘 주산지 바이러스 감염실태 조사
 적요
 인용문헌

저자정보

  • 최학순 HakSoon Choi. 국립원예특작과학원 채소과
  • 정봉남 Bong-Nam Chung. 국립원예특작과학원 원예특작환경과
  • 곽용범 Yong-Bum Kwack. 국립원예특작과학원 남해출장소
  • 정명일 Myeung-Il Jeong. 국립원예특작과학원 도시농업연구팀
  • 장석우 Suk-Woo Jang. 국립원예특작과학원 채소과
  • 양은영 Eun-Young Yang. 국립원예특작과학원 채소과
  • 조명철 Myeong-Cheoul Cho. 국립원예특작과학원 채소과
  • 정승룡 Seung-Ryong Cheong. 국립원예특작과학원 채소과

참고문헌

자료제공 : 네이버학술정보

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