원문정보
Anti-oxidant Function of Genistein against H2O2-induced Oxidative Stress in HaCaT Keratinocytes
초록
영어
The nutri-cosmetics was defined as a nutraceuticals which can exert the function, beauty and the structure of the skin. Genistein has been reported to possess potent pro-apoptotic, anti- inflammatory, and anti-oxidant properties. However, the physiological role and its underlying molecular mechanisms of genistein in H2O2- treated HaCaT keratinocytes are unknown. In this study, we examined the cellular anti-oxidant activity of genistein against H2O2-induced oxidative stress in HaCaT keratinocytes. The cellular anti-oxidant activity of genistein was assessed in HaCaT cells as judged by the 2’7’-dichlorofluorescein -diacetate (DCFH-DA)- based assay and image analysis using fluorescence microscopy. Genistein dose- dependently and effectively ameliorate the H2O2-induced oxidative stress of HaCaT keratinocytes. We further revealed that the cellular anti-oxidant activity of genistein mediated by activating of nuclear factor erythroid-2 -related factor 2 (Nrf2), the central transcriptional factor for expression of phase II detoxifying enzymes such as heme oxygenase-1 (HO- 1). Taken together, our results indicate that the activation of Nrf2 signaling is required for cellular anti-oxidant activity of genistein. These findings provide the scientific evidences for the development of Nrf2 targeted nutricosmetics.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 연구재료
2. HaCaT keratinocytes 배양
3. HaCaT keratinocytes에 대한 genistein의 세포독성
4. H2O2로 산화적 스트레스가 유도된 HaCaT keratinocytes에서 genistein의 항산화 활성
5. 현광현미경 이미지 분석을 통한 genistein의 항산화 활성
6. mRNA 발현 분석을 위한 PCR
7. 통계분석
Ⅲ. 연구결과 및 고찰
1. HaCaT keratinocytes에서 genistein의 세포독성
2. H2O2로 산화적 스트레스가 유도된 HaCaT keratinocytes에서 genistein의 항산화 활성
3. 형광 현미경 이미지 분석을 통한 genistein의 항산화 활성분석
4. Genistein 처리가 HaCaT keratinocytes의 Nrf2 및 HO-1유전자 발현에 미치는 영향
Ⅳ. 결론
참고문헌