원문정보
피인용수 : 0건 (자료제공 : 네이버학술정보)
초록
영어
UV spectrophotometer was used to detect protein-DNA complex from DNA melting profile under constant temperature increase. Melting temperature (Tm) was 43oC in copA duplex DNA alone. In the presence of Proteus mirabilis transcription regulator protein (PMTR) protein at 0.2 and 0.4 μM, Tm’s were 45 ± 0.5 and 47.6 ± 0.6oC, respectively. According to fluorescence polarization and gel shift assay. PMTR:copA complex was detected by the retarded migration on gel and the dissociation constant (Kd) was (9.2 ± 2.8) × 10-9 M.
목차
Abstract
1. Introduction
2. Experimental Section
2.1. Protein Purification
2.2. Optical Density Measurement
2.3. Fluorescence Polarization
3. Results and Discussion
4. Conclusion
Acknowledgements
References
1. Introduction
2. Experimental Section
2.1. Protein Purification
2.2. Optical Density Measurement
2.3. Fluorescence Polarization
3. Results and Discussion
4. Conclusion
Acknowledgements
References
저자정보
참고문헌
자료제공 : 네이버학술정보