원문정보
Characterization and Antibacterial Activity of Lactobacillus casei HK-9 Isolated from Korean Rice Wine, Makgeolli
초록
영어
The purpose of this work was to examine the antibacterial activity derived from a lactic acid bacterium isolated from korean rice wine, called makgeolli. Various physiological and biochemical properties of this strain were characterized. Both the BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Lactobacillus casei HK-9, and registered in GenBank as [JQ951606]. Growth rate, production of organic acids (e.g., lactic acid and acetic acid), and pH changes during growth were monitored. The maximum concentrations of lactic acid and acetic acid were approximately 576 mM and 199 mM, respectively, and pH was changed from 7.00 to 3.74 after 72 h of incubation. HPLC was used to confirm the production of lactic acid and acetic acid. Significant antimicrobial activity of the concentrated supernatant was demonstrated against various food-poison causing bacteria (e.g., Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Salmonella enteritidis). Ethanol tolerance of L. casei HK-9 showed up to 12% of ethanol within the culture.
목차
1. 서론
2. 재료 및 방법
2.1. 균주확보와 배양조건
2.2. 분리세균의 형태학적 관찰 및 생화학적 특성조사
2.3. 16S rRNA 염기서열의 계통수 분석
2.4. 세균의 생장에 따른 pH의 변화와 유기산 분석
2.5. 세균의 농축 배양상등액 제조
2.6. 농축 배양상등액의 항균활성 조사
2.7. 알코올 농도별 생존력 확인
3. 결과 및 고찰
3.1. 세균의 분리 및 특성
3.2. 세균의 동정 및 16S rRNA 염기서열의 계통수 분석
3.3. 세균의 생장에 따른 pH의 변화와 유기산 분석
3.4. 농축 배양상등액의 항균활성 조사
3.5. 알코올 농도별 생존력 확인
4. 결론
감사
References
저자정보
참고문헌
- 1On, G. U. (2011) Liquor column-2011 Economic prospect and liquor industry. Korea Alcohol and Liquor Industry 31: 14-21.
- 2Chun, J. W, C. W Ma, and K. H. Oh (2005) Physiological characterization of Lactobacillus sp. JK-8 isolated from shrimp aquaculture pond. Kor. J. Microbiol. 41: 18-23.
- 3Ringo, E. and F. J. Gatosoupe (1998) Lactic acid bacteria in fish: a review. Aquaculture 160: 177-203.
- 4Balcázar, J. L., D. Vendrell, I. de Blas, I. Ruiz-Zarzuela, J. L. Múzquiz, O. Gironés (2008) Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish. Aquaculture 278: 188-191.
- 5Song, Y. J., S. H. Park, J. Y. You, Y. S. Cho, and K. H. Oh (2009) Antibacterial activity against food-poisoning causing bacteria and characterization of Lactobacillus plantarum YK-9 isolated from kimchi. KSBB J. 24: 273-278.
- 6Baek, H., H. R. Ahn, Y. S. Cho, and K. H. Oh (2010) Antibacterial effects of Lactococcus lactis HK-9 isolated from feces of a new born infant. Kor. J. Microbiol. 46: 127-133.
- 7Ahn, H. R., J. S. So, and K. H. Oh (2011) Characterization and antimicrobial activity of lactic acid bacteria isolated from vaginas of women of childbearing age. Kor. J. Microbiol. 47: 308-315.
- 8Hirayama, K. and J. Rafter (2000) The role of probiotic bacteria in cancer prevention. Microbes Infect 2: 681-686.
- 9Wollowski, I., G. Rechkemmer, and B. L. Pool-Zobel (2001) Protective role of probiotics and prebiotics in colon cancer. Am. J. Clin. Nutr. 73: 451-455.
- 10Herich, R. and M. levkut (2002) Lactic acid bacteria, probiotics and immune system. Vet. Med. 47: 169-180.
- 11Aly, S., A. T. Cheik, H. N. Imael, and S. T. Alfred (2004) Antimicrobial activities of lactic acid bacteria strains isolated from burkina faso fermentated milk. J. Nutri. 3: 174-179.
- 12Yang, B. G., J, G. Lee, and M. S. Heo (2003) Antibacterial activity of Lactobacillus sakei BK19 against fish pathogenic bacteria. Kor. J. Microbial. 39: 56-61.
- 13Jeong, J. W., P. W. Nam, S. J. Lee, and K. G. Lee (2011) Antioxidant activities of Korean rice wine concentrates. J. Agric. Food Chem. 59: 7039-7044.
- 14Seo, M. Y., S. Y. Chung, W. K. Choi, S. H. Park, M. J. Seo, J. K. Park, J. W. Kim, and C. S. Park (2009) Anti-aging effect of rice wine in cultured human fibroblasts and keratinocytes. J. Biosci. Bioeng. 107: 266-271.
- 15Verschuere, L., G. Rombaut, P. Sorgeloos, and W. Verstraete (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol. Mol. Biol. Rev. 64: 655-671.
- 16The use of probiotics in shrimp aquaculture네이버 원문 이동
- 17Ma, C. W., Y. S. Cho, and K. H. Oh (2009) Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK-8 and JK-11. Aquaculture 287: 266-270.
- 18Schillinger, U. and F. K. Lucke (1989) Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55: 1901-1906.
- 19Maragkoudakis, P. A., K. C. Mountzouris, D. Psyrras, S. Cremonesem, J. Fischer, M. D. Cantor, and E. Tsakalidou (2009) Functional properties of novel protective lactic acid bacteria and application in raw chicken meat against Listeria monocytogenes and Salmonella enteritidis. Int. J. Food Microbiol. 130: 219-226.
- 20Biotechnology Journal 2012 Cover Gallery네이버 원문 이동