원문정보
Saccharification and Ethanol Production from Chlorella sp. Through High Speed Extrusion Pretreatment
초록
영어
Among various pretreatment processes for bioethanol production, extrusion pretreatment, one of cheap and simple process was investigated to efficiently produce fermentable sugars from micro alga, Chlorella sp. The biomass was pretreated in a single screw extruder at five different barrel temperatures of 45, 50, 55, 60 and 65℃, respectively with five screw rotation speed of 10, 50, 100, 150 and 200 rpm. The pretreated biomass was reacted with two different hydrolyzing enzymes of cellulase and amyloglucosidase since the biomass contained different types of carbohydrates, compared to cellulose of agricultural by-products such wheat and corn stovers, etc. In general, higher glucose conversion yield was obtained as 13.24 (%, w/w) at 55℃ of barrel temperature and 100 rpm of screw speed conditions. In treating 5 FPU/glucan of cellulase and 150 Unit/mL of amyloglucosidase, ca. 64% of cellulose and 40% of polysaccharides in the micro alga were converted into glucose, which was higher yields than those from other reported data without applying an extrusion process. 84% of the fermentable sugars obtained from the hyrolyzing processes were fermented into ethanol in considering 50% of theoretical maximum fermentation yield of the yeast. These results implied that high speed extrusion could be suitable as a pretreatment process for the production of bioethanol from Chlorella sp.
목차
1. 서론
2. 재료 및 방법
2.1. 실험재료
2.2. 고속압출기를 이용한 Chlorella sp.의 가수분해
2.3. Chlorella sp.의 당 성분의 분석
2.4. 고속압출 전처리 잔유물의 효소처리
2.5. 가수분해물의 에탄올 발효
2.6. Variable Pressure Field Emission Scanning ElectronMicroscopy (VP-FE-SEM)을 통한 Chlorella sp.의 입자표면 관찰
3. 결과 및 고찰
3.1. Chlorella sp.의 고속압출기를 이용한 가수분해
3.2. 전처리 잔유물의 효소 당화 공정
3.3. 가수분해물의 에탄올 발효
4. 결론
References