원문정보
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초록
영어
We prepared the polyclonal antibody anti-20α-hydroxysteroid dehydrogenase (anti-20α-HSD) against the recombinant full-length protein bovine 20α-HSD in Escherichia coli. The specificity of anti-20α-HSD was demonstrated using Chinese hamster ovary (CHO) cells transfected with recombinant bovine 20α-HSD and bovine placental tissues. According to western blot analysis, anti-20α-HSD specifically recognizes the 37-kDa protein bovine 20α-HSD. The protein is not present in untransfected CHO cells. Anti-20α-HSD also recognizes a specific protein in the ovaries and placenta of other animals. Immunostaining was used to detect expression of bovine 20α-HSD protein in the cultured luteal cells during the estrous cycle later.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Materials
Tissues
Preparation of Bovine 20α-HSD cDNA
Cells and Transfections
Production of Polyclonal Antibody
Bovine CL Cell Culture
Western Blot Analysis
RESULTS
DISCUSSION
REFERENCES
INTRODUCTION
MATERIALS AND METHODS
Materials
Tissues
Preparation of Bovine 20α-HSD cDNA
Cells and Transfections
Production of Polyclonal Antibody
Bovine CL Cell Culture
Western Blot Analysis
RESULTS
DISCUSSION
REFERENCES
저자정보
참고문헌
자료제공 : 네이버학술정보