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Purpose: This study aims at analyzing the effects of school health education through lessons on health knowledge, health attitude and health behavior among middle school students. Methods: The data was collected by self-administered questionnaires from the selected experimental group (n=136) and control group (n=148) in Seoul. Among them, 118 in experimental group and 121 in control group were included in final analysis. Experimental group took the 25- hour health education lessons from March 2010 to February 2011. The statistical method of analysis for homogeneity test of general characteristics was used x2 -test and for the effects of school health education through lessons was used unpaired t-test by SPSS/WIN 18.0 Program. Results: After the 25-hour health education lessons, statistical significance was observed between the experimental group and the control group to health knowledge. But two groups did not reach statistical significance on health attitude and health behavior. Conclusion: This study indicates that the school health education through lessons improves students' health knowledge. However in order to improve students' health attitude and behavior, it is necessary conduct more long-term school health education. Hence for the effective school health education, it is recommended to continue a long-term school health education and to secure a health education only classroom, textbooks, various educational materials and teaching methods and assistants.