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[Poster Presentation] - Gene Expression / Function

Interaction between Microtubule-associated Protein 1B and Glyceraldehydes 3-phosphate Dehydrogenase in Bovine Follicles

원문정보

Hyun-Min Tak, Changyong Choe, Young-Woo Cho, Jaehee Han, Dawon Kang

한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 36 No 2 Supplement 2012.06 pp.59-59
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in the brain development. MAP1B binds to many kinds of proteins directly or indirectly. In our previous studies, MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). This study was performed to examine interaction between MAP1B and GAPDH in bovine follicles using immunoprecipitation (IP) with western blot analysis and immunohistochemisty. MAP1B and GAPDH mRNA expression levels were down-regulated in bovine FCFs. Consistent with the semi-quantitative PCR data, their protein expressions were also down-regulated in FCFs. IP data showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting that these data might be resulted from a low level of MAP1B and/or GAPDH expression. These results suggest that GAPDH does not as always function as a loading control in bovine follicles.

키워드

저자정보

  • Hyun-Min Tak Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751 South Korea
  • Changyong Choe National Institute of Animal Science, RDA, Cheonan 330-801, South Korea
  • Young-Woo Cho Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751 South Korea
  • Jaehee Han Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751 South Korea
  • Dawon Kang Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751 South Korea

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