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Purpose: The purpose of this study was to identify relationship between knowledge and infection control behavior about Hepatitis B patient in health care -related majors. Methods: Data was collected from health care related majors- nursing, emergency rescue and dental hygiene with questionaire. Total respondents were 306 and we use all of them. We analysed students’ knowledge and infection control behavior about Hepatitis B patient and their relationship. Uni-variate analysis, t-test, ANOVA and correlation analysis test were conducted using SAS version 9.2. Results: Students who go the Hepatitis B lecture were significantly high Knowledge level about Hepatitis B(p<.05). Students who go to clinical education about Hepatitis B were significantly better behavior about infection control in clinical practice(p<.05). The relationship between knowledge about Hepatitis B and infection control in clinical practice was significantly negative(p<.05). Conclusions: It needs to be educated both infection control lectures and clinical practice to health care-related majors to prevent cross-infection between patients and health care providers.