earticle

영어

Although the studies of ingredient with antioxidant effect have been performing, in vitro and in vivo study of product containing antioxidant was not sufficient relatively. Potential antioxidants are very unstable due to auto-oxidation despite of their stabilized form exposed to air or sunlight. Especially, cosmetics were made through the process of high temperature, homogenization and mixture of a lot of ingredients. So, we cannot assure whether antioxidants keep the anti-oxidative effi cacy on the fi nished product without any confi rming test. In this study, we investigated antioxidant effect through DPPH and ORAC method and human volunteer test of three types of products with Actosome Ramalin as a stabilized form of Ramalin reported potent antioxidant previously. IC50 value of fi nished products, Essence, Ample and Cream, was 6.7%, 41.62% and 3.2% respectively. ORAC value showed 1034.52μM TE/g, 953.41μM TE/g and 1023.49μM TE/g. In in vivo human test of Essence, skin hydration, texture, brightness and lifting were statistically improved after 3 weeks and 6 weeks compared to before using product. In the case of Ample, skin elasticity of R5 parameter on the side of eye was statistically improved after 2 weeks and 4 weeks compared to before using product. Cream statistically improved skin texture and skin tone after 2 weeks and 4 weeks. Any problem in the skin was not revealed in human volunteer test. From these results, we suggest that we can claim the skin anti-oxidative effect of product in the demonstration of anti-oxidative effect of finished product. And products formulated only antioxidant as active ingredient were improved and alleviated phenomena of facial skin aging in human volunteer test. Therefore, anti-oxidative effi cacy demonstration of products containing anti-oxidant will be more practical and realistic approach to measure anti-oxidative level than raw material test.