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The purpose of our study is discovery of biomarker with elucidation of relationships between aberrant glycosylation and cancer. Glycosylation changes in the serum of cancer patients have been identified, and many of these can be derived from the induced cancer. In order to elucidate relationships between glycosylation and gastric cancer, we analyzed different glycosylation patterns of serum proteins of patients with gastric cancer compare to normal subjects. As results of blotting and LC/MS, we have been known that β-haptoglobin (β-Hp) is target glycoprotein which has 4 N-, 1O- glycosylation site for gastric cancer diagnosis. We analyzed glycosylation status of β-haptoglobin (β-Hp) purified from 10 cancer patients and 10 normal subjects. Lectin blotting index of serum b-haptoglobin with AAL was clearly higher for cancer patients than for healthy subjects. The results indicated that fucosylated glycan were increased in b-haptoglobin of gastric cancer patients than that of normal by and large. Increase in blotting index of b-haptoglobin of gastric cancer with PHAL which recognized tetra-structure due to high β1-6 GlcNAc blanching was also detected compare with that of healthy controls. Lectin blotting of b-haptoglobin with PHA-E, MAL, SNA was performed with some cases of cancer and healthy subjects, but the degree of this association was less than that for AAL and PHAL. We are now in the process of confirming these results with lager groups of cancer patients and other recent technology