earticle

영어

Capsosiphon fulvescens, a green alga, grows in copious amounts along the south-western coast of Korea and also widely throughout the world, including European and North american coasts. Although the C. fulvescens has been traditionally used in Korea as a functional food for centuries, the uses and potential values of bioactive materials from this species have not been well studied. The enzyme hydrolysates prepared with Alcalase (Novozyme, Denmark) was tested for ACE(Angiotensin converting enzyme) inhibitory effects. The maximum inhibitory activity was observed after Alcalase hydrolysis for 6hrs(72.9%). We separated of Alcalase hydrolysate by UF membrane system(Hydro-2K, Sartorius). The total carbohydrate of the below MWCO 2000 fraction was determined by the phenol-sulfuric acid method at 490 nm. The amount of sulfate residues was determined by Loui's method using Na2SO4 as a standard. Uronic acid content was determined by the carbazole reaction using D-glucuronic acid as a standard. Protein was quantified by Bradford method. The monosaccharide composition of the below MWCO 3000 was analyzed by HPLC after 2 M TFA hydrolysis and labeling of the hydrolysate with 1-phenyl-3-methyl-5- pyrazolone (PMP). Alcalase hydrolysate contained 67.1% total carbohydrate, 29.8% uronic acid, 0% sulfate and 3.1% protein. Below MWCO 2K contained 2.8% protein, 42.9% uronic acid, 0% sulfate and 54.3% total carbohydrate, which is mainly composed of glucose(95%).