earticle

영어

In this study, we evaluated the effects of the crude extract purities and pure paclitaxel contents on the efficiency and behavior (purity, yield, fractional precipitation time, precipitate shape and size) of fractional precipitation for the purification of paclitaxel from plant cell cultures. As the purity of crude extract and pure paclitaxel content were increased, the yield and purity of paclitaxel were increased and the precipitation time and precipitate size were decreased. Furthermore, we found that the maximum amount of pure paclitaxel content for fractional precipitation was 0.45~0.51% (w/v) regardless of purity of crude extract. By increasing the surface area per working volume (S/V) using a variety of ion exchange resins, fractional precipitation time could be reduced significantly as well as improvement of yield and purity of paclitaxel. Use of an ion exchange resin also resulted in the production of smaller paclitaxel precipitates since it inhibited the growth of particles.