earticle

영어

Vesosomes are widely used in the field of medicine, science, and engineering as model cells. However current techniques to create vesosomes are not up to date requiring long time (up to one day) and need expertise. Vesosomes are also difficult to control diameter, resulting in broad ranges of size distribution. Therefore, we demonstrate an automated platform of multiple materials-encapsulated vesosomes using microfluidic devices integrated with two phase T-junction systems. Water soluble fluorescently labeled liposomes are encapsulated into vesosomes. The entire system is integrated with specially designed microfluidic device, enabling full automation of vesosome formation with multiple encapsulants. The principles of automated vesosome formation employed a T-junction microfluidic device with two phase system, resulting in the monolayer of lipid along the interface. Emulsions with the lipid monolayer from the T-junction is introduced to another interface of a lipid monolayer. Specially designed microfluidic system enables this entire process automatable. To demonstrate that the vesicles generated from the device are vesosomes with multiple encapsulants, we show microscopic images from fluorescent microscope and confocal laser scanning microscope along with size distribution. Our work with vesosomes with multiple encapsulants will provide simple and efficient methods and apply to various fields of research including model cell/membrane studies.