earticle

영어

Controlled release technologies have been applied to intracellular delivery of DNA [1]. The DNA carriers have been engineered to release an intact DNA at the optimal time and to the targeted subcellular compartment [2]. In this report, fibroin microcapsule encapsulating DNA was prepared and investigated. The property of DNA release from the fibroin microcapsules was analyzed using shear fragmented DNAs and using different core materials. Linear DNA ranging from 3 – 10 kb was released but closed circular plasmids were not released from unmodified fibroin microspheres. Spatial distribution of the DNA in fibroin microspheres was also investigated. The release of DNA molecules, its size-dependency and cellular delivery was investigated.