earticle

영어

Development of a rapid, sensitive, and multiplex pathogen DNA sensor enables early diagnosis and subsequently proper treatment of infectious diseases, increasing the possibility of saving lives of infected patients. Here we report the development of an ultrasensitive and multiplex pathogen DNA detection method by combining patterned Au nanowire (NW)-on-film surface-enhanced Resonance Raman scattering (SERRS) sensor with exonuclease III-assisted target DNA recycling reaction. Combining the high sensitivity of NW-on-film sensor and the target recycling reaction significantly improved DNA detection performance, resulting in the detection limit of 100 fM corresponding to 3 amole. By positioning Au NWs at specific addresses, multiple pathogen DNAs could be identified in a single step. Clinical sample tests with multiple genomic DNAs of pathogens showed the potential of this sensor for practical diagnosis of infectious diseases. [This research was supported by WCU program through the Korea Science and Engineering Foundation funded by the Ministry of Education, Science and Technology]