earticle

영어

Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is known as a good molecular marker detection technique due to its sensitivity and reproducibility with conformation dependent separation of single strand DNA. For high resolution CE-SSCP analysis, new polymer matrix suitable for CE-SSCP, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer (PEO-PPO-PEO) was developed for separation of same-sized DNA fragments. The previous research showed improved resolution in the case of 15wt% condition of F108 polymer. In this research, effect of the heterogeneity in Pluronic polymer matrix was investigated with various blending method, ratio and length of each polymer. The two blending methods were used, Bi-molecular micelle blending and micelle mixture blending. As a result, heterogeneity generally reduced the resolution, except F108-F127 bi-molecular micelle blending which showed less heterogeneity in both blending method and polymer length. The result also implies that the character of micelle in the Pluronic polymer matrix is a critical factor, thus further optimization might give a better resolution.