earticle

영어

Type I collagen is the structural and functional scaffold in the extracellular matrix in skin and other tissues. The catabolic and anabolic process for collagen is in a highly regulated and balancing order, inclination in either the process leads skin disease or aging. MMP-1 is mainly responsible for degradation of the collagen-1, and the expression of MMP-1 is influenced by several factors (extrinsic of intrinsic). Thus regulation in MMP-1 action either by enzyme inhibitor or down regulation could solution for delayed aging. Our focus in the present study is to screen the non-active site binding inhibitor which specifically bind to MMP-1. To evaluate the binding affinity and inhibitor properties, we used Surface Plasmon Resonance based biosensor. The collagen peptide was immobilized on gold nanochip while MMP-1 was used in flow, and the response observed in response to BSA (control), and MMP-1-Inhibitor (Putative) was compared and the relative affinity was calculated. The results encourage us to screen a wide range of molecule having a similar skeleton to develop new class of anti-aging agent, through this SPR based biosensor as a label free high-throughput screening.