원문정보
초록
영어
Ionic liquids(ILs) have many advantages like low-volatility, Nonflammability and Electrical conductivity, so the ILs are widely used in various fields including extraction, sensors, refining materials and chemical catalysis. Although ILs are called to green solvent due to their high recovery rate, micro-spill to the environment is inevitable. In this work, we study Shewanella oneidensis MR-1 to elucidate the toxic mechanism of ILs to resolve problem. We assume that the major cause of cell death is the perturbation of cell membranes, resulting in the physical disruption of its structure and the interferences of signaling pathway. To further study the membrane interaction of ILs, we use a model sell membrane composed of amphiphilic biopolymer, polydiacetylen e(PDA). In particular well ordered PCDA monomers in a liposome are crosslinked by 1,4-addition by UV, showing red color with the naked eyes. To ensure the membrane perturbation of ILs we employ other analytical tools including differential scanning calorimetry(DSC) and particle size analyzer. We expect that our work will provide more comprehensive understandings of the effects of ILs to biological organisms, particularly in cells at a molecular level.