원문정보
초록
영어
Human adipose tissue-derived stem cells (ATSCs) can be differentiated into multiple mesodermal lineage cells through normal differentiation processes. They can be also trans-differentiated into some endodermal or ectodermal lineage cells through re-differentiation and de-differentiation processes. Here, we developed microfluidic culture system to induce neurogenic trans-differentiation potency of hATSCs. In this system, aggregates of neurospheres derived from hATSCs were trapped by arrays of micropillars in microchannels and cultured in neurogenic medium. After 3 day of the chip culture, HIF1α expression was significantly increased with upregulation of Oct4 and Nanog expressions, which indicated de-differentiation of hATSCs into more immature stem cells. After 7 days, cells actively spreaded out from the cell aggregates and started neural differentiation expressing TuJ-1. Then, they were fully differentiated into GABAergic neurons after 14 days. These results suggest that the microfluidic system can offer stem cells in-vivo like niches for cell proliferation as well as differentiation.