earticle

영어

Each odorant has its own odor fingerprint, a relationship between specific olfactory receptors and odorants, and human use this to percept certain scents. In this work, the odor fingerprints of three odorants, 1-octen-3-ol, trimethylamine, and 3-methyl-1-butanol, were discovered using artificial olfactory cells that each express diverse olfactory receptors on their membrane. The activation of artificial olfactory cells was identified using cAMP response element (CRE)-directed reporter gene assay which produce green fluorescence after external stimulation. The CRE-directed reporter gene assay method was optimized and subsequently used to screen the odor fingerprints of three selected odorants. 1-octen-3-ol, trimethylamine, and 3-methyl-1-butanol are indicators of fungal contamination, seafood spoilage, and salmonella contamination respectively. The discovered odor fingerprints will be a foundation on the development of biosensors applicable to food safety.