earticle

영어

For an early disease diagnosis, highly sensitive detection technology is necessary to analyze biomarkers quantitatively at low concentration. In case of prion which is a marker protein for mad cow disease, it is impossible to detect prion protein in serum using the conventional detection technology as the concentration is low. In this study, the orientation of antibodies is controlled using aptamer to improve sensitivity of a sensor. Antigens are analyzed by Real-time PCR measurements. +Targets to detect are Prostate specific antigen (PSA) and Escherichia coli O157:H7 (E.coli O157:H7). This system can be applied to disease diagnosis and environmental toxicity test. Two probes to capture antigens are magnetic microparticle (MMP) and gold nanoparticle (AuNP). Antibodies for antigens are immobilized on each particles. Using Fc-specific aptamer, antibodies are coupled with gold nanoparticle. An antigen is captured with magnetic probe and gold probe. After reaction, magnetic separation is carried out and washing steps are followed. This aptamer is sensitive to the change of pH. Gold nanopaticle coupled with aptamer is obtained by treatment of distilled water. Finally, antigens can be detected by Real-time PCR of aptamer. Acknowledgments: This research was a part of the project titled "Development of Sustainable Remediation Technology for Marine Contaminated Sediments" funded by the Ministry of Land, Transport and Maritime Affairs, Korea, This research was supported by by the R&D Program of MKE/KEIT(No. 10035578, Development of 2,3-butanediol and derivate production technology for C-Zero bio-platform industry), This work (research) is financially supported by the Ministry of Knowledge Economy(MKE) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology.