earticle

영어

In this study, cell chip was fabricated with conductive composite film composed of RGD peptide and carbon nanotubes (CNTs) for improving conductivity of RGD peptide film on gold electrode. The topological characteristics of modified electrode surface were validated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Human embryo kidney (HEK293T) cells were immobilized on fabricated cell chip to detect electrochemical characteristics by cyclic voltammetry. The electrochemical sensitivity of fabricated electrode evidently increase compared with bare gold and RGD film coated electrode. Thereafter, 2 kinds of nephrotoxic antibiotics were treated to HEK293T cells which were immobilized on modified electrode in cell chip. The electrochemical signal intensities linearly decreased with increase of antibiotics’ concentration. These results were confirmed by MTT assay. Our newly developed renal cell chip can be used in drug development field as label-free screening device to detect nephrotoxic effects of antibiotics on kidney. Acknowledgement: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2012-0000163) and by the Nano/Bio Science &Technology Program (M10536090001-05N3609- 00110) of the Ministry of Education, Science, and Technology (MEST), and by Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology.