earticle

영어

In order to achieve highly sensitive biosensors and electrochemical sensors, we require specialized materials with superior biocompatibility, physical-chemical durableness and stability. The diamond thin film is an excellent candidate for such biosensors and electrochemical sensors since it has low background current, biocompatibility, wide potential window (3.0~3.5 V), and physical-chemical stability. Diamond thin film will be enables to the selective and quantitative detection of biomolecule in vivo such as DNA, proteins, and cell. In this work we culture the nerve cell (SH-SY5Y) on diamond thin film surface to evaluate the biocompatibility. We investigated the effect of diamond thin film by cytotoxicity and biocompatibility with cultured human nerve cells by using MTT, calcein AM and DAPI. Human nerve cell exhibit the 110% cell viability on microdiamond compared with cell culture dish by MTT assay. Fluorescence data with calcein AM and DAPI exhibit 100% human nerve cells were detected onto microdiamond thin film, which is similar to MTT data. It has demonstrated that human cells proliferated on microdiamond thin film with very high viability and microdiamond material had no toxicity to cultured SH-SY5Y cells. We conclude that microdiamond thin film is a biological compatible material for potential cell culture matrix and bio-medical applications.