원문정보
초록
영어
Cell therapy has the potential for providing new way of clinical treatments. Mesenchymal stem cells (MSCs) are important tools for cell therapy because of their high proliferation and differentiation ability as well as immunosuppressive properties. MSCs are commonly cultured as monolayers, but there are indications that they lose cell-specific properties with multiplication in vitro. In addition, it was reported that the culture of MSCs as a spheroid form in a short period improved their therapeutic potential by increased expression of genes such as CXCR4 to promote adhesion to endothelial cells or IL-24 that has tumor suppressing properties. In this study, we developed a new three-dimensional (3D) culture system for periosteum-derived progenitor cells (PDPCs) which are one of diverse MSCs. The spheroid formation of PDPCs was induced by using a rotation platform. The spheres was found to maintain self-renewal ability. After 3D culture, the spheres expressed MSCs-specific surface markers and stem-cell-associated genes. In conclusion, an economical culture system could be developed. This system can be utilized for the scale-up 3D culture of the PDPCs.