원문정보
초록
영어
To become stem cell therapy available as a product, efficient industrial cultivation method is required. From this point of view, threedimensional (3D) culture is inevitable. Mesenchymal stem cells (MSCs) are attractive cell sources because they do not have any ethical problem and are safe from tumor formation. In previous studies, we accomplished the induction of periosteum-derived progenitor cells (PDPCs), known as a kind of MSCs, to insulin-producing cells (IPCs) in two-dimensional (2D) culture. In addition, we applied our induction method to 3D culture system using ultra-low attachment plates with different cell densities. In this study, we investigated the viabilities of the inducted cells on diverse cultivation methods. Time course viabilities were measured by WST-1[2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)- 2Htetrazolium] assay. Furthermore, we detected the functionalities of the cells by Enzyme-linked immunosorbent assay (ELISA) to measure the expression levels of insulin. In conclusion, it was found that PDPCs formed compact cellular spheroids and remained viable in 3D induction culture. It was also found that 3D culture was more effective than 2D culture for the differentiation of PDPCs to IPCs.