원문정보
초록
영어
Embryonic stem cells (ESCs) give rise to the possibility that stem cell therapy. However, before cell replacement therapies can be used, the problems with the tumorigenicity of ESCs must be solved. In the present study, we attempt to find biomarkers related with tumor formation of ESCs. We induced neuronal differentiation from H9, a hESCs, using a five-stage method and performed characterization of hESCs in each developing stages. We decided stage 3 as the step of cell sorting and performed separating cells on SSEA3 and CD133 using the MACS. In the results of in vivo assay with each sorted cells, CD133 positive and negative cells were not related with tumorigenesis. But groups injected the SSEA3 positive cells showed high levels of tumor forming rate as 100%, otherwise the group injected SSEA3 negative cells not detected tumorigenesis during periods of study. In these results, we recognized that SSEA3 related with undifferentiated status has important role in reduction of tumor formation on hESCs tumorigenesis, suggesting that these appears to be a useful biomarker for the assessment of the safety of stem cell-based therapy products using hESC.