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Introduction. The neuronal differentiation of bone marrow mesenchymal stem cells(BM-MSCs) has great potential for cellular therapies and might be useful in the treatment of diseases such as stroke or spinal cord injury. Many researches have investigated the effects of electromagnetic fields on neuronal differentiation of stem cells. Materials and Methods. Here, we analyzed the combined effects of ELFEMF(extremely low frequency electromagnetic fields) and differentiation medium (contatin hydrocortisone, insulin, forskolin, valproic acid, and KCl) on neural induction in human BM-MSCs. To induce neuronal differentication, BM-MSCs were incubated in differentiation medium for 1 day and treated for 7 days with 50 Hz, 100Hz, and 200 Hz electromagnetic fields subsequently. The cellular proliferation (MTT assay) investigated at different time points. Results. The results showed that exposure to neural differentiation medium and EMF decreased the cellular proliferation and enhanced the cellular differentiation. And we also examined immunocytochemistry, western blotting. We detected a significant increase of neuronal markers such as NF-L , Map2, and NeuroD1. Cells which were treated with 100 Hz EMF showed higher expression of NeuroD1, NF-L , and Map2 than 50 and 200 Hz. It confirmed by immunocytochemical analysis. And then, we confirmed that mean calcium current density increased after ELFEMF stimulation at 12 day of differentiation. We also found that ELFEMF increase the CREB phosphorylation at 24h of differentiation. Conclusion. Taken together, treatment of ELFEMF can be a good tool for neuronal differentiation and clinical implications.