earticle

영어

In an era of increasing oil prices and climate concerns, biodiesel has gained attention as alternative energy sources. Biodiesel derived from microalgae is a potential renewable and carbon neutral alternative to petroleum fuels. One of the most important decisions in obtaining oil from microalgae is the choice of algal species. A total of 32 microalgae cultures were isolated from an AMD (Acid Mine Drainage) in South Korea. 5 microalgae isolates were selected based on their morphology and ease of cultivation under our test conditions. The name of microalgae Nephroselmis sp. KGE 1, Nephroselmis sp. KGE 2, Uronema sp. KGE 8, Scenedesmus obiquus KGE 9 and Micractinium sp. KGE 10, based on 28S rRNA (D1D2 forward primer 5’-AGCGGAGGAAAAGAAACTA-3’,D1D2 revers primer 5’- TACTAGAAGGTTCGAT TA GT C-3’) sequence analyses. Nephroselmis sp. KGE 2 maximum growth of biomass concentration of 1.45±0.15 g dwt L-1 with a relatively lipid content of 20±0.5% w/w. Also, the fatty acids compositions of the studied species were mainly myristic, palmitic, palmitoleic, oleic, linoleic, g-linolenic, and linolenic acids. High concentration of oleic acid was also found in Nephroselmis sp. KGE 2, which led us to conclude that fatty acids of microalgae incubated in growth media were more effective for biodiesel production due to relatively lower saturation degree compared to the other cultures algal species. Our results suggest that Nephroselmis sp. KGE 2 can be a possible candidate species to produce oils for high quality biodiesel.