earticle

논문검색

포스터 발표 : 환경생물 및 에너지

Heterologous Expression and Characterization of acetyl xylan Esterase2 from Phanerochaete chrysosporium

원문정보

Nguyen Duc HUY, Ae-Yong MO, Hyoun-Young KIM, Seung-Moon PARK

한국생물공학회 한국생물공학회 학술대회 2012 춘계학술대회 및 국제심포지움 2012.04 pp.221-221
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

We cloned the cDNA of acetyl xylan esterase 2 (pcAXE2) gene from Phanerochate chrysosporium and expressed it into Pichia pastoris. The results indicated that the recombinant PcAXE2 (rPcAXE2) was efficiently produced and secreted, reaching at 938 Ul-1 after a 4 day induction. Molecular mass of the rPcAXE2 on SDS-PAGE was an approximately 63 kDa under hyperglycosylation. A biochemical analysis showed that rPcAXE2 has an optimum pH and temperature of 7.0 and 30-35oC, respectively, whereas specific activity, Km, and Vmax were 39.86 Umg-1, 92.2 μmol and 7.4 μmol min-1 ml-1, respectively. Moreover the rPcAXE2 also catalyzed the synthesis of peracetic acid (PAA) from ethyl acetate and hydrogen peroxide. The synthesized amount of PAA was 1.45 mM using 10 μg of rPcAXE2, which could be applied to the pretreatment of a cellulosic biomass in situ.

키워드

저자정보

  • Nguyen Duc HUY Division of Biotechnology, College of Environmental and Bioresource sciences, Chonbuk National University, Iksan,570-752,Republic of Korea.
  • Ae-Yong MO Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.
  • Hyoun-Young KIM LED-Agribio Fusion Technology Research Center, Chonbuk National University, Iksan 570-752, Korea.
  • Seung-Moon PARK Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.