earticle

영어

Some parts of algal polysaccharide in brown seaweed have been used as a source of health food and pharmaceutical agents.However, the remaining materials are usually wasted. To reutilize such waste, six novel strains were isolated from marine environment, and the isolated six strains clearly possessed laminarinase activity. However, high activity of laminarinase is required for the conversion of laminarin into useful oligosaccharides, tri-, di- and monosaccharides to save cost and time for saccharification. For this purpose, chemical mutation for the six strains were executed. The six cells were mutated using 30 mgl/ml of menadione, or L-ethionine, or aunomycin for 20 min at room temperature. Then, cells were washed twice with 0.85% saline. Through the plate assay, a better activity was confirmed from the mutated cells. The study for stability of the mutated cells is underway for effective degradation of laminarin.