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Recently, the beer industry utilizing mineral-rich bedrock water and malt barley (Hordeum vulgare, cv 'Baek Ho') is getting a lot of attention in Jeju island. This study focused on the usage of brew slurry which is left out after making beer, obtained from the pilot plant in Jeju Specia Self-Governing Province Development Corporation (JPDC). A 12-week feeding trial was conducted to investigate the effect of brewer's yeast (JBY) as a feed additive for juvenile olive flounder(Paralichthys olivaceus). The fish were fed four experimental dietary supplemented with 0% (control), JBY (1%, 3%, 5%) of BY mixed with basal diet. All the cases fed diets containing JBY (1%, 3%, 5%) of JBY revealed slightly higher survival rate than that of control experiment(0%). This study also compared their the glucose, cholesterol, glutamic oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT). The case of dietary 1% JBY was proven to be the optimal concentration in all parameters tested. The results of these experiments suggest that the brewer's yeast (JBY) can be a good candidate for dietary supplementation on the growth of juvenile olive flounder.