earticle

영어

Photosynthetic microalgaes, living in the ocean, use a specific mechanism called carbon concentration mechanism (CCM), which is promoting capacity to fix CO2 of Rubisco [1]. In this process of CCM, Carbonic Anhydrase (CA) plays a significant role as its reversible production of CO2 or bicarbonate. Chaetoceros neogracile, an antarctic diatom, is a primary producer of the polar region. The full genomic sequences is unknown yet, but our laboratory have generated the expressed sequence tag (EST) library [2]. In our study, we searched CA genes from the constructed EST library of C. neogracile aligning with other diatom, Phaeodactylum tricornutum and Thalassiosira pseudonana. From this process, we found a partial sequence of predicted gamma type CA and obtained full length cDNA clone as CnγCA. The putative CnγCA composes of 200 amino acid in length and is supposed to be secreted to cytosol. We are able to obtain soluble expressed CnγCA to purify recombinant CnγCA, and assay CnγCA activity.