earticle

영어

Microalgae are prokaryotic or eukaryotic photosynthetic microorganisms that can grow rapidly and live in harsh conditions due to their unicellular or simple multicellular structure. Microalgae can produce wide variety of valuable compounds which include the antioxidants, polyunsaturated fatty acids, oil, natural dyes and other fine chemicals and biomasses. Coccomyxa sp. C-169 is one of the microalgal species which live in freshwater. Whole genome sequence of Coccomyxa sp. C-169 has already been published by JGI and it can be applied to strain improvements to produce the valuable compounds. These experiments were carried out in order to investigate significant factors for enhancement of biomass production. In this experiment, we applied the FFD (Fractional Factorial Design) methods to N8 medium components to analyze the major factors that influence on cell growth. We choose the four factors from N8 medium (KNO3, KH2PO4 & Na2HPO4, MgSO4․7H2O, CaCl2․ 2H2O) and these four factors has divided into two levels with the eight experimental groups include the two centeral points. Experimental design has designed with MINITAB software and result of experiments was calculated using MINITAB software according to its compositions of medium and fermentation data.