원문정보
초록
영어
Diatoms are single celled algae that make silica shells (frustules) with nanoscale features imbedded within two-dimensional pore arrays. Living diatom itself metabolically insert nano-structured titanium dioxide into its surface. Navicula sp. (#1271) form Korean culture bank (KMMCC) was cultured in natural sea water supplemented with f/2 nutrient s in the photo bioreactor .In stage I , diatom cells grown up on dissolved silicon until silicon starvation was achieved. In stage II , soluble titanium and silicon were continuously fed to the silicon starved cell suspension (105 cells /ml) for 10 hrs. The feeding rate of titanium was designed to circumvent the precipitation of titanate in the liquid medium, and feeding rate of silicon was designed to sustain one cell division .The addition of titanium to the culture had no detrimental effect on the cell growth and preserved the frustule morphology. Intact frustule was prepared from harvest and analyzed for titanium using SEM, TEM, EDS and XRD analysis.