earticle

영어

A marine bacterium possessing alginate-degrading activity was isolated from brown seaweed soup liquefied by salted and fermented anchovy, previously. The bacterium was deginated to Sphingomonas sp. Strain MJ-3 based on 16S-23S ITS region sequences, biochemical characteristics and cellular fatty acid composition analysis. A novel alginate lyase gene was overexpressed in E. coli BL21 (DE3). The MJ-3 alginate lyase protein shared below 27.0% sequence identity with exolytic alginate lyase, A1-IV of Sphingomonas sp. A1. The time-dependent degradation of alginate by MJ-3 alginate lyase was analyzed by by high-field 1H nuclear magnetic resonance (NMR) spectroscopy, using an ECX-NMR 400Hz JEOL spectrometer (JEOL, USA). Based on the results of FPLC, TLC and NMR, the recombinant MJ-3 alginate lyase is determined to be an exolytic alginate lyase that can degrade the alginate into alginate monosaccharides. Acknowledgement : This work was supported by New & Renewable Energy R&D program(20093020090020) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology under the Korea Ministry of Knowledge Economy(MKE).