earticle

영어

Spore-forming bacteria are being used as probiotic supplements for human and animal use. Their low pH stability and ability to survive the gastric barrier makes them attractive as probiotic supplements. In this present study, BCNU 9028 strain was screened from meju, a Korean fermented soybean food starter. Biochemical, physiological characteristics and 16S rRNA sequence analyses indicate that strain BCNU 9028 belongs to the genus Bacillus. Bacillus sp. BCNU 9028 showed 92% survivability at pH 2.5 and could also withstand 0.3% oxbile. Furthermore, it was postulated that Bacillus sp. BCNU 9028 could prevent biofilm formation and adherence of foodborne pathogens such as Listeria monocytogenes, S. aureus and E. coli on the basis of its autoaggregation and coaggregation capacity with foodborne pathogens. It was showen that BCNU 9028 have good abilities to adhere to intestinal tract from its hydrophobic character (63.3%). Especially Bacillus sp. BCNU 9028 strain elicited antibacterial activity against both Gram-positive and Gram-negative pathogens. These findings suggested that Bacillus sp. BCNU 9028 strain could be used as potential probiotics. (Supported by the Korean Ministry of Education, Science Technology (MEST) and the National Research Foundation of Korea (NRFK) through the Human Resource Training Project for Regional Innovation)