earticle

영어

We have screened Paenibacillus sp. BCNU 5012 strains from various environments for the development of probiotics which can be survived in the presence of acid and bile salts, and inhibit human pathogens. Paenibacillus sp. BCNU 5012 isolate was a Gram- positive, gelatinase-negative, urese negative rod. On the basis of the results from 16S rDNA sequence comparison, it is found that Paenibacillus sp. isolate is phylogenetically most closely related to Paenibacillus polymyxa. Paenibacillus sp. isolate showed the highest degree of relatedness to the type strain of Paenibacillus polymyxa, sharing 99% 16S rDNA sequence similarity. When Paenibacillus sp. BCNU 5012 was subjected to acid tolerance test, it was found to survive up to 91.89% after 3 hr culture in pH 2.5 and 94.04% after 3h culture in pH 3.0. Paenibacillus sp. BCNU 5012 also showed excellent bile acid tolerance. In the inhibition test toward pathogenic microorganisms, Paenibacillus sp. isolates showed strong antimicrobial activity toward various kinds of bacteria. On the basis of the data presented above, Paenibacillus sp. isolates was found to have excellent potential as probiotics. (Supported by the Korean Ministry of Education, Science Technology (MEST) and the National Research Foundation of Korea (NRFK) through the Human Resource Training Project for Regional Innovation)