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One of the most well known Clostridia, Clostridium beijerinckii is a gram positive, rod-shaped, strictly anearobic, and motile bacterium. C. beijerinckii has been receiving industrial interest for its solvent, such as acetone, butanol and ethanol (ABE), known as an alternative biofuel. There has been considerable efforts to produce solvents more economically. However, neither titer nor productivity was high enough to be commercialized. Joint Genome Institute by DOE revealed the whole genome description of C. beijerinckii NCIMB 8052 in 2007. It became easier for metabolic engineers to manipulate biosynthetic pathways of this strain. In this study, we have tried to develop an effective gene-transfer protocol for this strain. Parameters, such as field strength, composition of electroporation buffer, and concentration of dna that influence the efficiency were examined.