earticle

영어

We engineered synthetic pathway for the production of 1-butanol in Escherichia coli by using 2-ketoisovalerate as an intermediate. The strain development was started from L-valine producing strain of E. coli, in which all the known negative regulations were removed and the carbon flux towards L-valine production was increased, the novel pathway for the biosynthesis of 1-butanol from 2-ketoisovalerate, the direct precursor of L-valine, was further amplified. The resulting engineered E. coli strain was able to produce 118 mg/L 1-butanol by microaerobic batch culture. These results suggest that an efficient production of 1-butanol is possible by properly assembling the synthetic metabolic pathways in E. coli. [This work was supported by the Advanced Biomass R&D Center of Korea (ABC-2010-0029799) through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST). Further supports by EEWS program of KAIST, and the World Class University program (R32-2008-000-10142-0) of the MEST are appreciated.].