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There have been some reports on sugar transport system of yeasts including S. cerevisiae, P. stipitis and Candida species. These transport systems are composed of both high affinity and low affinity for sugar including glucose and xylose. Generally, cells uptake glucose first then other sugar because of their availability. This is the reason why xylose uptake is limited during fermentation of glucose/xylose mixtures. Heterologous two xylose transporter genes, At5g 59250 and At5g17010 from Arabidopsis thaliana were codon optimized for functional expression in Candida tropicalis. A promoter region of glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) was isolated from the genomic DNA of C. tropicalis ATCC 20913 to construct a constitutive expression cassette. The codon-optimized xylose transporter genes were placed under the control of GAPDH promoter and integrated into the genome of C. tropicalis LXU1 which is xyl2-disrupted and NXRG(codonoptimized Neurospora crassa XR) introduced mutant. The resulting recombinant yeast, C. tropicalis LXT2-3(a At5g17010 gene introduced mutant), showed high volumetric productivity (1.08g/l·h) in the fed batch fermentation. This value is 35% higher than that of the parent strain, respectively.